ISOLATION, CHARACTERIZATION AND ANTIBACTERIAL SREENING OF ANTIBIOTICS PRODUCED FROM STREPTOMYCES ISOLATED FROM DUMPSITE SOILS IN ILORIN, NORTH CENTRAL NIGERIA
Article Sidebar
Main Article Content
Abstract
Abstract
Antibiotics are one of the most exploited metabolites produced by soil actinomycetes. This study isolated fifteen actinomycetes (A1 – A15) from dumpsite soils within Ilorin metropolis & screened them for antibacterial activity. Isolates were identified with morphology & biochemical characteristics.Their activity against clinical bacteria (Escherichia coli, Pseudomonas aeruginosa, Klebsiella pneumoniae & Staphylococcus aureus) was determined. Their bioactive metabolites after fermentation was assayed for by agar well diffusion & compared with reference antibiotics. Molecular analysis of two most active isolates was done. The most active metabolite was subjected to GCMS. All isolates were identified as Streptomyces, 7 isolates exhibited activity against 3 test bacteria with highest (10.5mm±0.35) by Streptomyces griseoplanus while S. aureus demonstrated total resistance to all isolates. Secondary activity revealed 7 of the metabolites as effective against the bacteria with highest activity (28.5±1.04mm) by S. sparsogenes showing similar activity with Cefuroxime (28.01±0.01mm). The two most active isolates were Streptomyces griseoplanus strain NR725RL-ISP 5009 & Streptomyces sparsogenes strain NBRC 1308616S & RNA nucleotide similarity revealed close phylogenic relationship. GC-MS revealed10 compounds in S. sparsogenes strain NBRC 1308616S metabolite. Streptomyces from dumpsite soils within Ilorin produced bioactive compounds against clinical test bacteria and thus could be potential sources of antibiotics.
Downloads
Article Details
Transfer of Copyrights
- In the event of publication of the manuscript entitled [INSERT MANUSCRIPT TITLE AND REF NO.] in the Malaysian Journal of Science, I hereby transfer copyrights of the manuscript title, abstract and contents to the Malaysian Journal of Science and the Faculty of Science, University of Malaya (as the publisher) for the full legal term of copyright and any renewals thereof throughout the world in any format, and any media for communication.
Conditions of Publication
- I hereby state that this manuscript to be published is an original work, unpublished in any form prior and I have obtained the necessary permission for the reproduction (or am the owner) of any images, illustrations, tables, charts, figures, maps, photographs and other visual materials of whom the copyrights is owned by a third party.
- This manuscript contains no statements that are contradictory to the relevant local and international laws or that infringes on the rights of others.
- I agree to indemnify the Malaysian Journal of Science and the Faculty of Science, University of Malaya (as the publisher) in the event of any claims that arise in regards to the above conditions and assume full liability on the published manuscript.
Reviewer’s Responsibilities
- Reviewers must treat the manuscripts received for reviewing process as confidential. It must not be shown or discussed with others without the authorization from the editor of MJS.
- Reviewers assigned must not have conflicts of interest with respect to the original work, the authors of the article or the research funding.
- Reviewers should judge or evaluate the manuscripts objective as possible. The feedback from the reviewers should be express clearly with supporting arguments.
- If the assigned reviewer considers themselves not able to complete the review of the manuscript, they must communicate with the editor, so that the manuscript could be sent to another suitable reviewer.
Copyright: Rights of the Author(s)
- Effective 2007, it will become the policy of the Malaysian Journal of Science (published by the Faculty of Science, University of Malaya) to obtain copyrights of all manuscripts published. This is to facilitate:
(a) Protection against copyright infringement of the manuscript through copyright breaches or piracy.
(b) Timely handling of reproduction requests from authorized third parties that are addressed directly to the Faculty of Science, University of Malaya. - As the author, you may publish the fore-mentioned manuscript, whole or any part thereof, provided acknowledgement regarding copyright notice and reference to first publication in the Malaysian Journal of Science and Faculty of Science, University of Malaya (as the publishers) are given.
You may produce copies of your manuscript, whole or any part thereof, for teaching purposes or to be provided, on individual basis, to fellow researchers. - You may include the fore-mentioned manuscript, whole or any part thereof, electronically on a secure network at your affiliated institution, provided acknowledgement regarding copyright notice and reference to first publication in the Malaysian Journal of Science and Faculty of Science, University of Malaya (as the publishers) are given.
- You may include the fore-mentioned manuscript, whole or any part thereof, on the World Wide Web, provided acknowledgement regarding copyright notice and reference to first publication in the Malaysian Journal of Science and Faculty of Science, University of Malaya (as the publishers) are given.
- In the event that your manuscript, whole or any part thereof, has been requested to be reproduced, for any purpose or in any form approved by the Malaysian Journal of Science and Faculty of Science, University of Malaya (as the publishers), you will be informed. It is requested that any changes to your contact details (especially e-mail addresses) are made known.
Copyright: Role and responsibility of the Author(s)
- In the event of the manuscript to be published in the Malaysian Journal of Science contains materials copyrighted to others prior, it is the responsibility of current author(s) to obtain written permission from the copyright owner or owners.
- This written permission should be submitted with the proof-copy of the manuscript to be published in the Malaysian Journal of Science
References
Abbas KH., Al-Snaf AE. &Bander KI. (2009). Isolation and Identification of Antibiotics Produced by Penicillium brasilium Batista Isolated from Salahaddin Province Soils. Thi-Qar Medical Journal (TQMJ) 3: 71-87.
Abdulkadir M. & Waliyu S. (2012). Screening and isolation of the soil bacteria for ability to produce antibiotics. European Journal of Applied Science 4(5): 211-215.
Adegboye MF. & Babalola OO. (2013). Isolation, characterization and antibacterial activity of Streptomycetes from rhizosphere soils in North West Province, South Africa. The Asian International Journal of Life Sciences 9: 403-421.
Ahmed RN., Abdullahi MA., Zakariyah RF., Gambari-Ambali RO., Adeyemi SB., Afonja AI. & Adebisi OO. (2015). Antibacterial Action of Silver Nano Particles of Extract of Thevetia nerifolia. International Journal of Phytofuels and Allied Sciences4(1): 31-55.
Ajijolakewu K., Leh C., Wan AbduLLah W. & Lee C. (2016). Assessment of the Effect of Easily-metabolised Carbon Supplements on Xylanase Production by Newly Isolated Trichoderma asperellum USM SD4 Cultivated on Oil Palm Empty Fruit Bunches. BioResources11(4): 9611-9627. doi:10.15376/biores.11.4.9611-9627
Aliero AA., Emmanuel E., Josephat MN., Sambo HA., Matilda AO. & John O. (2017). Antibacterial Activity of Actinomycetes Isolated from Waste Dump Soil from Western Uganda. Microbiology Research Journal International 21(5): 1-14.
Ashok KP. & Karpagam P. (2016). Screening of Antibiotic Producing Actinomycetes from the Sediments of Undisturbed Forest Areas and Its Hyper Activity after Mutation. International Journal of Advances in Chemical Engineering and Biological Sciences 3(2): 46-43.
Bizuye A., Moges F. & Andualem B. (2013). Isolation and screening of antibiotic producing actinomycetes from soils in Gondar town, North West Ethiopia. Asian Pacific Journal of Tropical Diseases 3(5): 375-381.
Bizuye A., Bii C., Erastus G. & Maina N. (2017). Antibacterial metabolite prospecting from actinomycetes isolated from damped soils from Thika central part of kenya. Asian Pacific Journal of Tropical Diseases 7(2): 757-764.
Boeck LD., Christy KL. & Shah R. (1971). Production of anticapsin by Streptomyces griseoplanus. Applied microbiology 21(6): 1075–1079.
Chaudhary HS., Yadav J., Shrivastava AR., Singh S., Singh AK. & Gopalan N. (2018). Antibacterial activity of actinomycetes isolated from different soil samples of Sheopur (A city of central India). Journal of Advanced Pharmaceutical Technology and Research 4(2): 118-123.
Das RK., Brar SK. & Verma M. (2016). Fumaric Acid: Production and Application Aspects. Platform Chemical Biorefinery 8: 133-157.
Dinos PG. (2017). The Macrolide Antibiotics Resistsnce. British Journal of Pharmacology 174: 2967-2983.
Emami E., Issazadeh K., Akhavan SA. & Tajehmiri A. (2017). Isolation and Characterization of Aerobic Actinomycetes from Soil in Northern Iran and Evaluation of their Antimicrobial Potential J Med Bacteriol 6(3 &4): 14-22.
Fawole MO. & Oso BA. (2007). Laboratory manual of microbiology, 5th edition spectrum book limited, Ibadan, Nigeria, pp. 16-33.
Fay GD. & Barry AL. (1974). Methods for detecting indole production by gram-negativenon-spore forming anaerobes. Applied Microbiology 27(3): 562-565.
Kaur S., Kaur HP. & Kaur G. (2016). Isolation and characterization of antibiotic producing actinomycetes from agriculture soil. World Journal of Pharmacy and Pharmaceutical Sciences 5(6):1109-1117.
Njenga WP., Mwaura FB., Wagacha JM. & Gathuru EM. (2017). Methods of isolating actinomycetes from the soils of Menengai crater in Kenya. Archives of Clinical Microbiology 8: 3.
Manganyi MC., Tchatchouang CK., Regnier T., Bezuidenhout CC. & Ateba CN. (2019) Bioactive Compound Produced by Endophytic Fungi Isolated from Pelargonium sidoides Against Selected Bacteria of Clinical Importance. Mycobiology 47(3): 335-339.
Mary PA. & Giri RS. (2017). GC-MS Analysis of Bioactive Compounds of Acryranthes aspera. World Journal of Pharmaceutical Research 7(1): 1015-1056.
Ohnishi Y., Ishikawa J., Hara H., Suzuki H., Ikenoya M., Ikeda H., Yamashit A., Hattori M. & Horinouchi S. (2008). Genome sequence of the streptomycin- producing microorganism Streptomyces griseus IFO 13350. Journal of Bacteriology 190(11): 4050-4060.
Raja A. & Prabakarana P. (2011). Actinomycetes and Drug-An Overview. American Journal of Drug Discovery and Development 1: 75-84.
Sathi ZS., Rahman MAA. & Gafur MA. (2001). Identification and in vitro antimicrobial activity of compound isolated from Streptomyces species. Pakistanian Journal of Biological Science 4: 1523-1525
Sharma P., Pajni S., Dhillon N., Vadehra D. & Dube D. (1986). Limitations of the Congo-Red staining techniques for the detection of cellulolytic activities. Biotechnology letters 8(8): 579-580.
Sharma M., Dangi P. & Choudhary M. (2014). Actinomycetes: Source, Identification, and Their Applications. International Journal of Current Microbiology and Applied Sciences 3(2): 801-832.
Sikander A., Maria N., Aiman TL., Maryam S. & Attia M. (2018). Production of Microbial Metabolites and Optimization of Key Factors Involving their Hyperproduction in Batch Culture. European Journal of Pharmaceutical and Medical Research 5(5): 80-88.
Simon C. & Daniel R. (2011). Metagenomic analyses: past and future trends. Applied and Environmental Microbiology 77(4): 1153-1161.
Singh AP., Singh RB. &Mishra S. (2012). Studies on isolation and characterization of antibiotic producing microorganism from industrial waste soil sample. The Open Nutraceutical Journal 5: 169-172.
Tango CN., Mansur RA. & Oh DH. (2015). Fumaric Acid and Slightly Acidic Electrolyzed Water Inactivate Gram Positive and Gram Negative Foodborne Pathogens. Microorganisms 3(1): 34-46
Zhang Hongyu., Qiuling Zhou., Tingting Lou., Suying Wang. & Haihua Ruan. (2017). Draft genome sequence of broad-spectrum antibiotic sparsomycin-producing Streptomyces sparsogenes ATCC 25498 from the American Type Culture Collection. Journal of Global Antimicrobial Resistance 11: 159-160.
Ziemert N. & Jensen PR. (2012). Phylogenetic approaches to natural product structure prediction. Methods in enzymology517, 161–182.